Savita Pahwa, M.D., Director
spahwa@med.miami.edu
305-243-7732
Sion Williams, PhD, Co-Director
slwilliams@med.miami.edu
305-243-3875
Margie Roach Ph.D., Lab Manager
MRoach@med.miami.edu
305-243-4822
Jessica Salinas, B.S. MBA, Lab Manager
JSalinas@med.miami.edu
305-243-3550
Service request:
https://redcap.miami.edu/surveys/?s=FRJL83YYR7
Integrated Bioinformatics Network (IBN). RNA-Seq and bioinformatics with publication ready figures
Isolation and cryopreservation of human PBMC, T cells, B cell subsets and other cell types
Evaluation of cytokines and soluble mediators in plasma, serum or supernatants by ELISA, Luminex mutliplex beads (Magpix), or ultrasensitive Simoa technology (Quanterix)
Flow cytometry assays (4-15 color staining) including intracellular staining and T and B cell phenotype and function
ELISPOT (up to 2 color) or FLUOROSPOT (up to 4 color) assays to detect single cell cytokine/antibody secretion from B cells or T cells
Gut microbial translocation marker evaluation including LPS, soluble CD14, soluble CD163 and intestinal fatty acid binding protein (iFABP)
Biorepository development for investigator-initiated studies. Repository management using Freezerworks or LDMS system for liquid nitrogen/ultralow freezer storage of cells, plasma, PAXgene samples of RNA or DNA.
To provide elutriated human monocytes – can be used for replication assays, establishment of macrophage and DC cultures
Macrophage/DC culture platforms – the price for culturing macrophage or DC cultures would be double the price of ordering $40 per million
siRNA/shRNA services – aid in design of optimal procedures for gene silencing in primary cells
Viral cDNA intermediates – for reservoir analysis
Primary cell replication assays – to aid in assessment of antiviral agent efficiency
Digital PCR – for quantification of viral reservoirs 3-5 million PBMCs, or 2-3 million purified CD4 cells
HIV-1 infectivity assays – for assessment of viral fitness
Viral Tropism assays – for assessment of macrophage and T cell tropism
RNA and DNA extraction from clinical samples for viral reservoir analysis
High density qPCR using Fluidigm Biomark HD with the ability to handle low input samples
Single cell gene expression by qPCR using Fluidigm C1 and Biomark HD platforms.
Single cell gene expression by NGS using BD Genomics Precise 3'Whole Trasncriptome Amplification (3'WTA) and targeted panels, or single cell RNA-Seq using Fluidigm C1 with Clontech Smarter V4 chemistry
Project design and oversight for next generation sequencing and collaborative bioinformatics.
