Molecular Modeling and Drug Discovery Core Laboratory: Provides investigators with priority services to help translate their basic science discoveries to clinical therapies. The small molecules obtained from structure-based and/or cheminformatics approaches can also be used as the chemical probes to decipher the complex biomedical mechanisms.
Simon Wang, PhD, Ph:202-806-6547, x.simon.wang@gmail.com
The Core Lab develops custom codes for high-throughput virtual screening and scoring functions. We maintain a screening libraries collection of over 17.5 million commercially available compounds, categorized into specialized subsets including drug-like libraries, targeted libraries, diversity libraries and natural product libraries.
The laboratory offers expertise and hardware/software support for other biocomputations such as the atomistic simulation techniques in Molecular Dynamics, QM/MM, Monte Carlo and Comparative Modeling. It also provides custom organic synthesis for lead optimization and novel small molecules upon request.
The laboratory hosts most of the major commercial molecular modeling packages.
A specialized visualization system for biomolecular graphics and a group of high-volume data storage systems.
Virus Detection and Analysis Laboratory: Virus Detection and Analysis Molecular Virology Core Laboratory provides services related to analysis of various steps of HIV replication in target cells and the effects of anti-HIV compounds and treatments. We can evaluate stages of reverse transcription, integration, transcription, and viral release, analyze cell-to-cell transmission, and isolate virus from primary infected cells.
Michael Bukrinsky, MD, PhD, Ph:202-994-2036, mbukrins@gwu.edu
The lab provides virus detection and analysis services. Services include RT and p24 assays, in vitro infections, RT-PCR and PCR, virus infectivity assay, fusion assay, Western blot analysis for various HIV proteins and co-immunoprecipitation assays for HIV-related proteins.
RCMI Proteomics Core Laboratory: The RCMI Proteomics Core Laboratory, located at Howard University Interdiciplinary Research Building Room 315, provides services associated with analysis of an entire complement of proteins related to a particular process. The laboratory runs several research projects and provides collaborative consolidation of instrumentation, technical expertise, and support personnel to enhance the impact and availability of mass spectrometry and other analytical techniques.
Sergei Nekhai, PhD, Ph:202-865-4545, snekhai@howard.edu
Protein Identification Analysis: including peptide sequencing and protein modificaiton sites identificaiton such as phosphorylation, ubiquitination and methylation etc. by nano-LC-MS/MS and database query using Proteome Discoverer 2.0 ($20/hour, HPLC-MS/MS running time is 2 hours for each sample)
Label-free quantitative analysis of protein samples using SIEVE 2.1 (Thermo Scientific) Cost: $120/Sample
Phosphoproteomics Analysis: including sample preparation, digest, enrichment using TiO2 and Fe-NTA kits and nano-LC-MS/MS analysis of none-enriched, enriched on TiO2 and enriched on Fe-NTA portions of the original samples) Cost: $400/Sample
Molecular Weight Analysis (High Resolution) of Small molecules Cost: $25/Sample
Training of Protein Sample Preparation for LC-MS Analysis: Step-by-step teaching to self prepare digested protein samples for LC-MS/MS. Cost: $200/Training Session
Molecular Modeling and Drug Discovery Core Laboratory: provides investigators with priority services to help translate their basic science discoveries to clinical therapies. The small molecules obtained from structure-based or cheminformatics approaches can also be used as the chemical probes to decipher the complex biomedical mechanisms.
X. Simon Wang, PhD, Ph:202-806-6547, x.simon.wang@gmail.com
custom codes for high throughput virtual screening and scoring functions. It maintains a screening library collection of over 17.5 million commercially available compounds as well as specialized collections including drug-like libraries, targeted libraries, diversity libraries and natural product libraries.
The laboratory offers expertise and hardware and software support for other biocomputations such as the atomistic simulation techniques in Molecular Dynamics, QM/MM, Monte Carlo and Comparative Modeling. It also provides custom organic synthesis for lead optimization and small molecule chemical probes upon request.
The laboratory supports most of the major commercial molecular modeling packages
A specialized visualization system for molecular graphics and a group of high-volume data storage systems.
NextGen Sequencing Core Laboratory: The DC CFAR NGS Core Laboratory, based in Dr. Jordan’s Laboratory at The George Washington University, School of Public Health in the Science and Engineering Hall, provides NextGen Sequencing assays on the MiSeq NGS instrument for HIV and HCV for the DC CFAR investigator community.
Jeanne A. Jordan, PhD, Ph:202-994-7062, jajordan@gwu.edu
• Free Consultation Services• Targeted NGS of 4 regions within HIV-1 including PR, RT, IN and full-length ENV, representing ~56% of the HIV-1 genome. We also have the capability of analyzing the 46 drug resistant mutations (DRM) recognized by WHO. • WGS of HCV• RNA-Seq of host cells
Douglas F. Nixon, MD, PhD, Core Director
dnixon@gwu.edu
202-994-3532
Sergei Nekhai, PhD. Core Co-Director
snekhai@howard.edu
202-865-4545
Farah Mouhanna, Core Manager
mouhanna_farah@email.gwu.edu
