Douglas F. Nixon, MD, PhD, Core Director
dnixon@gwu.edu
202-994-3532
Sergei Nekhai, PhD. Core Co-Director
snekhai@howard.edu
202-865-4545
Farah Mouhanna, Core Manager
mouhanna_farah@email.gwu.edu
Microscopy and Imaging Core Laboratory: provides the ability to prepare samples, collect images of live or fixed cells and conduct live-cell time-lapse studies using well established techniques specific to HIV research.
Anastas Popratiloff, MD, PhD, Ph:202-994-2881, anastas@gwu.edu
Zeiss LSM 510: The Zeiss LSM 510 confocal system is equipped with fully motorized Zeiss Observer Z1 inverted microscope,
Zeiss LSM 710: The Zeiss LSM 710 confocal system (NIH 1S10RR025565) on AXIO EXAMINER upright microscope and LSM 710 with a 34 spectral detection channels and two transmitted light photomultipliers,
JEOL JEM-1200EX: The JEOL JEM-1200EX is a classic Transmission Electron Microscope (TEM) 100 kV equipped with 4 megapixel SIA-CAM.
Ultramicrotome workstations: Reichert Jung Ultracut E, Sorval MT5000 and MT6000 are available for sectioning specimens for TEM imaging. A Thermo Scientific Microm HM650 V slicer is available for living tissue.
Microscope Imaging Workstation: The microscope workstation has an Olympus BX-60 upright Fluorescence Microscope with a Ludl Focus Motor and an Evolution Digital CCD Camera connected to a computer with Image Pro Plus 6.2, including 3-D constructor module and ImageJ.
Nikon Eclipse TE300: The Nikon Eclipse TE300 inverted microscope with Optronics CCD camera at 1MP resolution can perform phase contrast, bright-field and fluorescence imaging. It is also available equipped with a microinjection system.
Image Analysis Workstation: The Image-Pro Plus v6.2 and Metamorph Image Analysis Workstation includes 3D constructor and deconvolution modules
Storm 860 Molecular Imager: The Storm 860 and personal densitometer with Image Quant software are available for blue and red lasers for excitation.
Georgetown University, The Center for Functional and Molecular Imaging: integrates advanced MRI, EEG and NIRS to study physiological changes associated with HIV/AIDS in research participants.d Molecular Imaging (CFMI)
John VanMeter, PhD, Ph:202-687-3592, jwv5@georgetown.edu
provides access to a 3T MRI Scanner, a stand-alone high-density EEG system and two Near Infrared Spectroscopy (NIRS) systems.
3T MRI Scanner: This instrument can perform functional MRI (fMRI) to examine neuronal activation using different cognitive paradigms; single voxel spectroscopy (SVS) and mutli-voxel spectroscopy to examine concentration of different metabolites in vivo; diffusion tensor imaging (DTI) to examine white matter tract integrity; and voxel-based morphometry (VBM) to examine changes in gray and white matter density.
EEG Laboratory:, is a fully equipped electrophysiology laboratory, which will allow experiments combining the superb temporal resolving capabilities EEG approaches with the sensitivity and spatial resolving properties of functional MRI. The integration of electrophysiology and other imaging modalities will allow investigation of research questions probing modulations in the spatiotemporal character of brain activity.
Near Infrared Spectroscopy: Near Infrared Spectroscopy (NIRS) is similar to fMRI and can be used to examine cortical activity based on hemodynamic changes especially in those subjects for whom MRI is not an option.
Research Pathology Core Laboratory: The Research Pathology Core Laboratory is available to provide histology and pathology services for DC CFAR clinical and basic science investigators. Our mission is to provide quality histological tissue preparation and processes in a timely and cost-efficient manner for research cell and tissue specimens, including frozen sections, tissue processing, embedding, sectioning, routine H&E and special stains, frozen sections and Immunohistochemistry. The lab can also provide expertise in-house to assist investigators in developing new histological approaches that can further their research productivity.
Patricia Latham, MD, Ph: 202-994-5057, platham@mfa.gwu.edu
Histology: Processing of cells and tissues to formalin-fixed paraffin-embedded (FFPE) specimens and tissue sections includes fixation, processing, embedding, and sectioning for routine histology. Routine H&E staining and special stains are available. Cryosectioning is provided for frozen tissue.
Immunohistochemistry: Services include manual immunohistochemical staining with established antibodies, and optimization of new antibodies, using a variety of detection systems. Immunofluorescent staining is
Necropsy: Assistance with gross examination, animal tissue harvest or specific tissue collection can be provided, with trimming of tissues as determined in consultation.
Pathology: Pathology consultative services are available for studies using human tissues and animal models, including recommendations on histologic methods and interpretations of pathology or immunological stains, as determined by an investigators need and available expertise.
Pathology: Pathology consultative services are available for studies using human tissues and animal models, including recommendations on histologic methods and interpretations of pathology or immunological stains, as determined by an investigators need and available expertise.
